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axioobserver z1 inverted fluorescent microscope  (Carl Zeiss)


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    Carl Zeiss axioobserver z1 inverted fluorescent microscope
    Axioobserver Z1 Inverted Fluorescent Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 4854 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axioobserver+fluorescence+microscope/Inverted+microscope+Axio+Observer+3/10__1021_slash_acsomega__5c13065-129-9-14
    Average 99 stars, based on 4854 article reviews
    axioobserver z1 inverted fluorescent microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: A Nanobody Interaction with SARS-CoV-2 Spike Allows the Versatile Targeting of Lentivirus Vectors
    Article Snippet: .. Slides were viewed on a Zeiss AxioObserver fluorescence microscope using a 63× (Planapochromat) objective and a Zeiss filter set 10 (excitation bandpass, 450–490; beamsplitter Fourier transform, 510; emission bandpass, 515–565) for green fluorophores, or Zeiss filter set 20 (excitation bandpass, 546/12; beamsplitter Fourier transform, 560; emission bandpass, 575– 640) for red fluorophores. .. Images were collected with Zeiss Axiovision software with a fixed gain setting of 100 and equivalent exposure times for all samples for each fluorophore in a given experiment.

    Article Title: Second site reversion of HIV-1 envelope protein baseplate mutations maps to the matrix protein
    Article Snippet: .. Samples were viewed on a Zeiss AxioObserver fluorescence microscope using a 63× (Planapochromat; NA = 1.4) objective and a Zeiss filter set 20 (excitation bandpass 546/12, beamsplitter Fourier transform 560, and emission bandpass 575-640). .. For cell surface staining analysis, images were collected with Zeiss Axiovision software with a fixed gain setting of 100 and a fixed exposure time of 50 ms. To determine percentages of surface staining, individual cells initially were traced out as ovals with Fiji (ImageJ; 77) software, and total cell brightness values were calculated from image areas times average brightness values.

    Article Title: Innervation of cranial muscles requires Mllt11/Af1q/Tcf7c function during trigeminal ganglion development
    Article Snippet: .. Microscopy was performed using a Zeiss AxioObserver fluorescence microscope equipped with an Apotome 2, 10x and 20x objectives, and Hamamatsu Orca Flash v4.0 digital camera. .. For whole mount immunostaining, microscopy was performed using a Zeiss V16 Zoomscope equipped with an Axiocam 506 mono digital camera.

    Article Title: Dichotomous Effects of Glypican-4 on Cancer Progression and Its Crosstalk with Oncogenes
    Article Snippet: .. Analysis of the fluorescent images was accomplished using an AxioObserver fluorescence microscope from Carl Zeiss equipped with a 63X/1.25 Oil M27 EC Plan-Neofluar objective and Axiocam 305 mono Camera. ..

    Article Title: A nanobody interaction with SARS-COV-2 Spike allows the versatile targeting of lentivirus vectors.
    Article Snippet: .. The slides were viewed on a Zeiss AxioObserver fluorescence microscope using a 63× (Planapochromat) objective and a Zeiss filter set 10 (excitation bandpass, 450–490; beamsplitter Fourier transform, 510; emission bandpass, 515–565) for green fluorophores, or Zeiss filter set 20 (excitation bandpass, 546/12; beamsplitter Fourier transform, 560; emission bandpass, 575–640) for red fluorophores. .. The images were collected with Zeiss Axiovision software with a fixed gain setting of 100 and equivalent exposure times for all samples for each fluorophore in a given experiment.

    Article Title: Alginate-Dialdehyde-Based Reporter Ink Enabling Online Detection of Matrix Metalloproteinase Activity of Encapsulated Cells.
    Article Snippet: Biofabrication and three-dimensional (3D) bioprinting enable precise spatial arrangement of cells within biomaterial scaffolds.. We developed an alginate-based and Förster resonance energy transfer (FRET)-responsive “turn-on” reporter ink platform to enable real-time monitoring of matrix metalloproteinase (MMP) activity.. Three distinct MMP-cleavable turnon peptide reporters were synthesized and characterized for their cell-specific cleavage profiles using recombinant MMPs, cellderived media, and different cell cultures (NIH3T3, HEK293, and MelHo).

    Article Title: Second site reversion of HIV-1 envelope protein baseplate mutations maps to the matrix protein.
    Article Snippet: The HIV-1 Envelope (Env) protein cytoplasmic tail (CT) recently has been shown to assemble an unusual trimeric baseplate structure that locates beneath Env ectodomain trimers.. Mutations at linchpin residues that help organize the baseplate impair virus replication in restrictive T cell lines but not in permissive cell lines.. We have identified and characterized a second site suppressor of these baseplate mutations, located at residue 34 in the viral matrix (MA) protein, that rescues viral replication in restrictive cells.

    Article Title: tANCHOR-cell-based assay for monitoring of SARS-CoV-2 neutralizing antibodies rapidly adaptive to various receptor-binding domains
    Article Snippet: To capture the bottom of the 96-well plate, it was placed on a Reflecta light plate (Intas, Göttingen, Germany), and the well bottom was imaged by using a SONY NEX5 digital camera with a SONY macro E3.5/30 objective (Sony, Tokyo, Japan). .. In order to analyze cell adherence of transfected cells the wells were imaged using an AxioObserver fluorescence microscope with an Axiocam 503 mono, filter cube for red dyes and a 20x objective (Zeiss). .. Covid-19 uninfected individuals in the groups receiving one, two, or three doses of Pfizer-BioNTech BNT162b2 mRNA vaccine were confirmed by reactivity test to nucleocapsid protein (NCP) conducted in parallel to cell-based ELISA and performed by using the same starting dilution of incubated sera.

    Microscopy:

    Article Title: A Nanobody Interaction with SARS-CoV-2 Spike Allows the Versatile Targeting of Lentivirus Vectors
    Article Snippet: .. Slides were viewed on a Zeiss AxioObserver fluorescence microscope using a 63× (Planapochromat) objective and a Zeiss filter set 10 (excitation bandpass, 450–490; beamsplitter Fourier transform, 510; emission bandpass, 515–565) for green fluorophores, or Zeiss filter set 20 (excitation bandpass, 546/12; beamsplitter Fourier transform, 560; emission bandpass, 575– 640) for red fluorophores. .. Images were collected with Zeiss Axiovision software with a fixed gain setting of 100 and equivalent exposure times for all samples for each fluorophore in a given experiment.

    Article Title: Second site reversion of HIV-1 envelope protein baseplate mutations maps to the matrix protein
    Article Snippet: .. Samples were viewed on a Zeiss AxioObserver fluorescence microscope using a 63× (Planapochromat; NA = 1.4) objective and a Zeiss filter set 20 (excitation bandpass 546/12, beamsplitter Fourier transform 560, and emission bandpass 575-640). .. For cell surface staining analysis, images were collected with Zeiss Axiovision software with a fixed gain setting of 100 and a fixed exposure time of 50 ms. To determine percentages of surface staining, individual cells initially were traced out as ovals with Fiji (ImageJ; 77) software, and total cell brightness values were calculated from image areas times average brightness values.

    Article Title: Innervation of cranial muscles requires Mllt11/Af1q/Tcf7c function during trigeminal ganglion development
    Article Snippet: .. Microscopy was performed using a Zeiss AxioObserver fluorescence microscope equipped with an Apotome 2, 10x and 20x objectives, and Hamamatsu Orca Flash v4.0 digital camera. .. For whole mount immunostaining, microscopy was performed using a Zeiss V16 Zoomscope equipped with an Axiocam 506 mono digital camera.

    Article Title: Dichotomous Effects of Glypican-4 on Cancer Progression and Its Crosstalk with Oncogenes
    Article Snippet: .. Analysis of the fluorescent images was accomplished using an AxioObserver fluorescence microscope from Carl Zeiss equipped with a 63X/1.25 Oil M27 EC Plan-Neofluar objective and Axiocam 305 mono Camera. ..

    Article Title: A nanobody interaction with SARS-COV-2 Spike allows the versatile targeting of lentivirus vectors.
    Article Snippet: .. The slides were viewed on a Zeiss AxioObserver fluorescence microscope using a 63× (Planapochromat) objective and a Zeiss filter set 10 (excitation bandpass, 450–490; beamsplitter Fourier transform, 510; emission bandpass, 515–565) for green fluorophores, or Zeiss filter set 20 (excitation bandpass, 546/12; beamsplitter Fourier transform, 560; emission bandpass, 575–640) for red fluorophores. .. The images were collected with Zeiss Axiovision software with a fixed gain setting of 100 and equivalent exposure times for all samples for each fluorophore in a given experiment.

    Article Title: Alginate-Dialdehyde-Based Reporter Ink Enabling Online Detection of Matrix Metalloproteinase Activity of Encapsulated Cells.
    Article Snippet: Biofabrication and three-dimensional (3D) bioprinting enable precise spatial arrangement of cells within biomaterial scaffolds.. We developed an alginate-based and Förster resonance energy transfer (FRET)-responsive “turn-on” reporter ink platform to enable real-time monitoring of matrix metalloproteinase (MMP) activity.. Three distinct MMP-cleavable turnon peptide reporters were synthesized and characterized for their cell-specific cleavage profiles using recombinant MMPs, cellderived media, and different cell cultures (NIH3T3, HEK293, and MelHo).

    Article Title: Second site reversion of HIV-1 envelope protein baseplate mutations maps to the matrix protein.
    Article Snippet: The HIV-1 Envelope (Env) protein cytoplasmic tail (CT) recently has been shown to assemble an unusual trimeric baseplate structure that locates beneath Env ectodomain trimers.. Mutations at linchpin residues that help organize the baseplate impair virus replication in restrictive T cell lines but not in permissive cell lines.. We have identified and characterized a second site suppressor of these baseplate mutations, located at residue 34 in the viral matrix (MA) protein, that rescues viral replication in restrictive cells.

    Article Title: tANCHOR-cell-based assay for monitoring of SARS-CoV-2 neutralizing antibodies rapidly adaptive to various receptor-binding domains
    Article Snippet: To capture the bottom of the 96-well plate, it was placed on a Reflecta light plate (Intas, Göttingen, Germany), and the well bottom was imaged by using a SONY NEX5 digital camera with a SONY macro E3.5/30 objective (Sony, Tokyo, Japan). .. In order to analyze cell adherence of transfected cells the wells were imaged using an AxioObserver fluorescence microscope with an Axiocam 503 mono, filter cube for red dyes and a 20x objective (Zeiss). .. Covid-19 uninfected individuals in the groups receiving one, two, or three doses of Pfizer-BioNTech BNT162b2 mRNA vaccine were confirmed by reactivity test to nucleocapsid protein (NCP) conducted in parallel to cell-based ELISA and performed by using the same starting dilution of incubated sera.

    Staining:

    Article Title: Alginate-Dialdehyde-Based Reporter Ink Enabling Online Detection of Matrix Metalloproteinase Activity of Encapsulated Cells.
    Article Snippet: Biofabrication and three-dimensional (3D) bioprinting enable precise spatial arrangement of cells within biomaterial scaffolds.. We developed an alginate-based and Förster resonance energy transfer (FRET)-responsive “turn-on” reporter ink platform to enable real-time monitoring of matrix metalloproteinase (MMP) activity.. Three distinct MMP-cleavable turnon peptide reporters were synthesized and characterized for their cell-specific cleavage profiles using recombinant MMPs, cellderived media, and different cell cultures (NIH3T3, HEK293, and MelHo).

    Transfection:

    Article Title: tANCHOR-cell-based assay for monitoring of SARS-CoV-2 neutralizing antibodies rapidly adaptive to various receptor-binding domains
    Article Snippet: To capture the bottom of the 96-well plate, it was placed on a Reflecta light plate (Intas, Göttingen, Germany), and the well bottom was imaged by using a SONY NEX5 digital camera with a SONY macro E3.5/30 objective (Sony, Tokyo, Japan). .. In order to analyze cell adherence of transfected cells the wells were imaged using an AxioObserver fluorescence microscope with an Axiocam 503 mono, filter cube for red dyes and a 20x objective (Zeiss). .. Covid-19 uninfected individuals in the groups receiving one, two, or three doses of Pfizer-BioNTech BNT162b2 mRNA vaccine were confirmed by reactivity test to nucleocapsid protein (NCP) conducted in parallel to cell-based ELISA and performed by using the same starting dilution of incubated sera.



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    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging <t>Microscope</t> with transillumination ( Trans ) or <t>fluorescence</t> illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.
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    Image Search Results


    (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging Microscope with transillumination ( Trans ) or fluorescence illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.

    Journal: bioRxiv

    Article Title: Membrane-anchored PrP Sc is the trigger for prion synaptotoxicity

    doi: 10.1101/2025.07.11.664221

    Figure Lengend Snippet: (A) Hippocampal neurons from ZH3 (Prnp -/- ) mice were transduced at DIV6 with a lentivirus encoding eGFP under control of the synapsin promoter to achieve neuron-specific expression. Cultures were then imaged on DIV21 using the EVOS Live Cell Imaging Microscope with transillumination ( Trans ) or fluorescence illumination ( GFP ). Merge shows a superposition of the two images. (B) Prnp -/- neurons were untransduced, or were transduced with lentiviruses encoding WT, G126V, or V208M PrP. Cells were fixed and stained with Alexa488-phalloidin (green) to visualize F-actin in dendritic spines and with D18 antibody (red) to detect PrP. Neurons from ZH3 (Prnp -/- ) and C57BL/6 (Prnp +/+ ) mice were used as negative and positive controls, respectively. Scale bar = 20 µm. (C) Higher magnification images of neurons showing dendritic shafts with protruding spines. Scale bar = 5 µm. (D) Dendritic spines of 12-15 neurons (each having 3-5 dendrites/neuron) from 2 independent experiments were counted in randomly selected areas, and statistical comparisons made using a one-way ANOVA multiple comparison test. Spine number is expressed per µm length of dendrite. Prnp -/- vs WT, p=0.4802; Prnp -/- vs G126V, p=0.9493; Prnp -/- vs V208M, p=0.2103. ns = not significant.

    Article Snippet: 10-15 neurons were randomly selected and imaged using a Zeiss AxioObserver D1 Fluorescence Microscope and/or a Zeiss LSM 700 Laser Scanning Confocal Microscope with 63X oil objectives.

    Techniques: Control, Expressing, Live Cell Imaging, Microscopy, Fluorescence, Transduction, Staining, Comparison